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For the statistics of stress reporter assay data, R (3.3.2) (R Core Team, 2017) and nlme (Pinheiro et al., 2009) (nlme: Linear and Nonlinear Mixed Effects Model) was used to perform a linear mixed effect model for longitudinal data with the following call: lme [value time treatment_dose, random = time | plateID, correlation = corAR1 (form = 1 |plateID)]

Purification performance of Glutathione 4FF resins. 50 mL of E.coli lysate containing a GST-tagged fusion protein was loaded on 0.5 mL packed Glutathione 4FF resins at 0.5 mL/mL. The column was then washed with 10 mL of PBS. The target GST-tagged protein was eluted with 7.5 mL of elution buffer (50 mM Tris-HCl, 10 mM GSH, pH 8.0). Fractions of lysate, flow-through, and eluate were analysed by SDS-PAGE. Lanes: (1) Raw cell lysate; (2) Flow-through; (3) Eluate

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