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doi: 10.1001/jama.285.11.1444

Vitamin B12 deficiency is most commonly seen in older adults, vegetarians and vegans, individuals with certain gastrointestinal disorders, and those who have had gastrointestinal surgery

BAC water added 1.0 mL Concentration 5 mg/mL (5,000 mcg/mL) 300 mcg dose 0.06 mL / 6 units 500 mcg dose 0.10 mL / 10 units BAC water added 2.0 mL Concentration 2.5 mg/mL (2,500 mcg/mL) 300 mcg dose 0.12 mL / 12 units 500 mcg dose 0.20 mL / 20 units BAC water added 3.0 mL (recommended for accuracy) Concentration ~1.667 mg/mL (1,667 mcg/mL) 300 mcg dose 0.18 mL / 18 units 500 mcg dose 0.30 mL / 30 units 3.0 mL is the most common research-context dilution because it pushes daily draws above 10 syringe units, which makes measurement on a 0.3 mL U-100 syringe easier

Additional experimental contexts include vascular inflammation, myocardial remodeling, neuroinflammatory paradigms, hepatic oxidative injury, antiviral immune signaling, and wound healing biology